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Define Ventures

VC

USHealthtechAI/MLB2B SaaSConsumerEnterprise

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Investment thesis

Define Ventures was founded on the belief that the future of healthcare will be defined by those who bring together a deep understanding of the healthcare ecosystem paired with a technology-driven mindset. The firm combines Silicon Valley principles with deep healthcare operating experience, investing at the incubation, seed, Series A, and Series B stages in companies that serve multiple ecosystem partners — consumers, payers, providers, employers, and life-sciences companies. As Lynne Chou O'Keefe describes it: "We're rebuilding the house of healthcare" — starting with the consumer gateway, through which people enter the health system, and extending into enterprise software, value-based care, and tech-enabled services. Define focuses on founders who possess both deep healthcare domain expertise and technical excellence, and actively supports portfolio companies through GTM strategy, talent placement, and follow-on capital introductions.

Notable quotes

We have this analogy, which is we're rebuilding the house of healthcare and that starts with what we call the front door or we'll call it a consumer gateway. How people enter into the health system, we believe, is changing, between health and wellness, mental behavioral health, and coming in and having those needs is rapidly evolving with the consumerization of healthcare.

Lynne Chou O'Keefe · MobiHealthNews Q&A — Define Ventures seeks to invest in digital health after $460M raise · 2023-04-18

Startups need to have real differentiation such as with data velocity and uniqueness, where the data creates a real moat. Large language models (LLMs) are not enough. Differentiation around data, trust, safety, accuracy are important.

Lynne Chou O'Keefe · MobiHealthNews Q&A — Define Ventures seeks to invest in digital health after $460M raise · 2023-04-18

We're starting to move further up the stack. AI was first used for isolated use cases in healthcare and life sciences; the technology is moving into an era of systemwide transformation. Define is investing in founders that have multiple products in the pipeline and can deploy them into multiple teams in an organization.

Chirag Shah · Fierce Healthcare — Define Ventures is molding the next generation of AI founders · 2025-07-22

Team

Lynne Chou O'Keefe

Founder & Managing Partner

HealthtechConsumerEnterpriseAI/ML

Chirag Shah

Partner

HealthtechB2B SaaSAI/MLEnterprise

Michael Wert

Partner

HealthtechAI/ML

Hong Truong

Principal

HealthtechConsumerEnterprise

Chuka Esiobu

Principal

Healthtech

Garrett Howard

Principal

Healthtech

Recent investments

CompanyRoundSizeDate
Hims & HersSeed/Early2018-01-01
Unite UsSeries B$35m2020-01-01
Cohere HealthSeries C$90m2025-05-14
FOLX HealthSeries A$30m2022-10-12
TiaSeries B2022-01-01
CentivoSeries C$75m2024-09-24
LuminaiSeries B$38m2026-04-09
9amHealthSeries A2022-01-01
Concert HealthSeries B$42m2022-04-28
Crescendo HealthSeed2023-01-25

Frequent co-investors

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